معرفی Cryptococcus adeliensis به عنوان عامل شانکر درختان میوه هسته‌دار در استان آذربایجان غربی

Canker disease of stone fruit trees which is one of the most serious and damaging diseases of these trees in the world, have been reported from many parts of the world with various causal agents such as fungi and bacteria. In recent years, yeast has been reported as stone fruits canker agent in some regions of Iran. In the spring of 2017, sampling of stone fruit trees was carried out to evaluate the canker caused by yeasts in the Oshnavieh region. After disinfestation of separated cankers and chopping in distilled sterile water, 50 μl of the suspension were streaked on the nutrient agar sucrose 2% medium. Yeast-like colonies were selected andscreened based on microscopic observations. The yaest-like colonies were circular and highlighted in light pink color on the YPGA medium and Spherical cells observed under microscope were about 8-5 micrometersPathogenicity of all yeast isolates were confirmed on apricot leaves and annual branches of peach trees (Elberta cultivar) and nectarine (Redgold cultivar) in vitro and garden. Identification of pathogenic strains was done by physiological, biochemical and molecular methods. Small subunit of ribosome RNA was amplified and sequenced by a pair of D1 / D2 primers . After editing, the sequences were compared with the Gene Bank (NCBI) and in order to confirm the identification, the phylogenetic tree was constructed using the MEGA6 software by Neighbor joining method. Yaest causal agent of stone fruit canker was identified as Cryptococcus adeliensis. this is the first report of canker caused by Cryptococcus adeliensis in West Azarbaijan province.