Bean (Vicia faba L.) is one of the most important legumes in Iran and many countries. Many of virus disease have been reported on broad bean that reduce the quantity and quality of this crop in the world. Among the viruses reported from broad bean, Bean yellow mosaic virus (BYMV) is one of the most common viral diseases that infects a wide range of plants. Bean yellow mosaic virus is from the Potyviridae family and the Potyvirus genus. In this research, 120 leaf samples of broad bean fields in Rudan of Hormozgan province were collected during 2017-2018 growing season. The collected samples were showed severe green mosaic and leaf malformation. In the first step, the samples were analyzed by DAS-ELISA test using polyclonal antibody. Samples with positive reaction were subjected to complementary study through reverse transcriptional molecular methods and reverse transcriptional polymerase chain reaction (RT-PCR) using specific primers for HC-Pro region of Bean yellow mosaic virus genome. The amplified region in the PCR reaction were evaluated on 1% agarose gel, which resulted in the replication of a DNA fragment with an expected length of 1100 bp in the collected samples. Analysis of the obtained sequences using the BLAST tools in NCBI and it comparison with available sequences in GenBank showed their 94-82% similarity with the previously reported sequences of Bean yellow mosaic virus (BYMV) from other parts of the world.This is the first report of the presence of Bean yellow mosaic virus (BYMV) from broad bean fields of Hormozgan Province.