According to the strategic importance of wheat in the country and the low average wheat yield compared to the global scale, it is necessary to offer solutions to increase wheat production in Iran. seed biopriming with probiotic bacteria can use to be effective approach to achieve this purpose, because seed bioprime can help to establishment of suitable population of bacteria on seed and, on the other hand, increase the rate and extent of germination of seed wheat Arranges. Seed bio priming is one of the seed priming methods that interconnects seed prior to planting with biological agents. The aim of this study was to evaluate the effect of wheat seed biopriming with probiotic bacteria Bacillus subtilis UTB96 and its effect on germination rate, wheat growth indices and the final yield on field. The results showed that biopremium expedite and increased germination by 35% compared to control treatment under field conditions. bioprime also increased wheat growth index such as leaf area index, wet weight and dry weight of the plant, Number of fertile tillers and weight gain of 1000 seeds of wheat were compared with the control treatment. In this study, seeds biopriming increased the absorption of essential elements by the plant and increased. also The final yield and harvest index of primed seeds increased by 34% and 7%, respectively. According to the results, it seems that biopremium can be a suitable way to increase wheat production per unit area. . This method is compatible with other management Programs and can solve the problem of using bio control agents that sometimes unstable behavior due to the inability to colonize in the presence of soil micro flora. On the other hand, the bio control agent is based with adequate population on the seed.