Bean root rot caused by soil pathogenic fungus, F. solani f.sp. phaseoli, is considered as one of the most important diseases of bean in the world and in Iran. It is very persistent disease in the soil and lasts for a long time in the soil, and its control is very difficult. Therefore, the use of other methods, especially the use of tolerant cultivars, can be useful in reducing the disease. In this study, the response of eight cultivars and 34 lines of red beans to the disease in greenhouse conditions was evaluated by contamination of root plants. In this study, the response of eight cultivars and 34 lines of red beans to the disease in greenhouse conditions was evaluated by inoculation of root plants. Samples of infected bean plants were collected from bean fields of Zanjan province and their pathogenicity was studied on bean plants. Among them, high virulent isolates were selected and its effect on the cultivars and lines of beans were examined. Experiment was conducted in a completely randomized design with three replicates and three observations in each replication under greenhouse conditions. The effect of infection with virulent isolate on growth factors of plant consisting foliage and root length, dried root and foliage weight, length of necrosis lesion on root, foliar disease severity and root disease severity was assayed. The results were showed that none of the cultivars had complete resistance to the disease and among the cultivars of red bean, Mahali cultivar was sensitive and Yaghout cultivar was tolerant to the disease.