Fusarium oxysporum f.sp. lycopersici is the causal agent of tomato vascular wilt and one of the important restrictive agents in the cultivation of this plant in the fields and greenhouses of East Azerbaijan province. This research was conducted for study on the effect of mycorrhizal fungus Glomus versiforme, antagonistic bacterium Bacillus methylotrophicus, fungicide Carbendazim and combined treatments of these biological and chemical agents on fungal pathogen at glasshouse conditions in two cultivars of tomato (Primo early and Falat). In the plants inoculated with Fusarium, amount of root colonization by mycorrhizal fungus was evaluated at 34.7 and 35.4% in Primo early and Falat cultivars respectively and these levels were elevated to 39.5 and 46.7% in presence of antagonistic bacterium (if bacteria apply 48 hours before pathogen). All three control agents could increase fresh and dry weight of roots and shoots, diameter and length of stems of treated plants in compare with positive and negative control plants. Regarding the severity of the disease, all treatments showed a significant difference with control plants at 5% of probability. In Primo early, combined treatments of antagonistic bacterium-Carbendazim and antagonistic bacterium-mycorrhiza (when bacteria and fungicide applied 48 hours before inoculation with the pathogen) showed the highest level of disease control at 88.34 and 84.46% respectively. In Falat, also Carbendazim and combined treatment of antagonistic bacterium-Carbendazim (if applied 48 hours before inoculation) caused 72.5% reduction in disease severity. Combined treatment of mycorrhiza-antagonistic bacterium could decrease disease severity in this cultivar in the amount of 30% in compare with infected control plants.