Rapeseed (Brassica napus L.) is one of the most important oil sources in the world. Pathogenic fungi reduce the yield of this product. In order to identify the fungi associated with root, crown and canola leaves in Lorestan province during 2017 years of canola fields in different regions of the province including the cities of Alashtar, Khorramabad, Veisian, Mamoulan and Poldokhtar, contaminated and suspected contaminated samples were collected and transferred to special lab bags. To isolate of isolates from root, crown and leaf tissues, fragments were prepared in half to one cm. After washing with ordinary water, they were disinfected with sodium hypochlorite 1% for 1 to 3 minutes and disinfected three times with sterilized disinfectant sterile surface sterilization and then on a PDA medium. After fungi growth, isolation and purification (by single sporing) were performed. A total of 74 fungal isolates were obtained. of the isolates, 39 isolates belonged to genus Alternaria(52.70%), 12 isolates belonging to the genus Fusarium (16.21%), 7 isolates belonging to the genus Ulocladium (9.45%), 7 isolates belonging to the genus Bipolaris (9.45%), 4 isolates belonging to the genus Penicellium (5.4%), 3 isolates belonging to the genus Aspergillus (4.05%), 2 isolates belonging to the genus Beuvaria (2.7 %). From these fungi, Alternairai (52.70%) had the highest frequency and Beuvaria (2.7 %) had the lowest abundance. These fungi were identified based on morphological characteristics using valid keys and papers. In this study, Beuvaria bassiana, U. atrum, U. chartarum, F. sambucinum and F. diversisporum species were identified. The above species are reported for the first time from Iran.